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Updated: Jun 13, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
Not lost in space: trafficking in the hedgehog signaling pathway
Ljiljana Milenkovic1, Matthew P Scott
1Howard Hughes Medical Institute, Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305-5439, USA.
None:
Compartmentalization within cells provides spatial organization of signaling pathways and ensures the specificity of signaling. In vertebrates, the primary cilium, a tiny microtubule-based protrusion present on most cells, is essential for organizing events during Hedgehog signal transduction. When cells are stimulated with Hedgehog ligands, proteins in the pathway move in and out of the cilia. Protein kinase A (PKA), which is implicated in diverse cellular processes including protein trafficking, is a component of the Hedgehog signaling pathway. PKA has been localized near primary cilia, at a location suitable for regulating the localization of other proteins in the pathway.
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