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Hematopoiesis in regenerated bone marrow within hydroxyapatite scaffold
Atsushi Fujita1, Makoto Migita, Takahiro Ueda
1Department of Pediatrics, Nippon Medical School, Tokyo 113-8602, Japan.
Pediatric Research
|April 15, 2010
Summary
Regenerated bone marrow (BM) on hydroxyapatite scaffolds can support and mobilize hematopoietic stem cells (HSCs). This regenerated BM acts as a portable source for HSC transplantation, reconstituting a functional niche in vivo.
Area of Science:
- Biomedical Engineering
- Hematology
- Stem Cell Biology
Background:
- The creation of a functional bone marrow (BM) niche is crucial for hematopoietic stem cell (HSC) support and regeneration.
- Hydroxyapatite (HA) scaffolds seeded with BM-derived stromal cells (BMSCs) offer a potential platform for BM regeneration.
Purpose of the Study:
- To investigate if regenerated BM on HA scaffolds can reconstitute a functional niche capable of supporting and mobilizing HSCs.
- To evaluate the potential of this regenerated BM as a portable source for HSCs.
Main Methods:
- Ly5.2 BMSCs on HA scaffolds were implanted subcutaneously into Ly5.2 recipient mice.
- Lineage-negative Ly5.1 BM cells expressing luciferase (luc) were administered intravenously.
- Secondary implantation of scaffolds into new recipients was performed, followed by G-CSF stimulation and bioluminescence detection.
Main Results:
- Luciferase-expressing cells were detected in scaffolds for up to 6 months post-secondary implantation.
- G-CSF injection led to widespread bioluminescence distribution, indicating HSC mobilization.
- Luc+ cells were emitted from scaffolds even after removal, demonstrating sustained HSC support and release.
Conclusions:
- Regenerated BM on HA scaffolds partially reconstitutes a functional niche.
- This regenerated BM can support HSCs and facilitate their delivery to native BM in vivo.
- Regenerated BM on scaffolds represents a promising portable source for HSCs.
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