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Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection
Jesse D McMullen1, Warren R Zipfel
1School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA.
Optics Express
|April 15, 2010
Summary
We designed a new microscope objective for multi-photon (MPM) and second harmonic generation (SHG) imaging. This objective enhances light collection, improving imaging performance with a large field of view.
Area of Science:
- Optical microscopy
- Microscope objective design
Background:
- Multi-photon microscopy (MPM) and second harmonic generation (SHG) are advanced imaging techniques.
- Existing microscope objectives can limit field of view and light collection efficiency.
Purpose of the Study:
- To develop a novel objective lens for MPM and SHG microscopy.
- To enhance the field of view (FOV) and light collection efficiency of microscopy objectives.
Main Methods:
- De novo design of a microscope objective.
- Integration of a dichroic beam splitter within the objective.
- Optimization for moderate numerical aperture (NA) and straightforward construction.
Main Results:
- The designed objective provides a large field of view (FOV).
- Incorporation of a dichroic beam splitter increased the front aperture.
- Enhanced solid angle of collected emission by an order of magnitude compared to existing designs.
Conclusions:
- The novel objective design offers improved light collection for MPM and SHG microscopy.
- This design facilitates enhanced imaging performance with a larger FOV.
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