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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 13, 2026

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
07:50

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Published on: September 30, 2021

Vidicon characteristics under continuous and pulsed illumination.

G W Liesegang, P D Smith

    Applied Optics
    |April 15, 2010
    PubMed
    Summary

    This study details interfacing a vidicon detector with a minicomputer for improved scan control and data collection. It characterizes vidicon performance under different lighting conditions and evaluates methods to reduce image lag.

    Area of Science:

    • Instrumentation
    • Optical detectors
    • Image processing

    Background:

    • Vidicon detectors are crucial for light detection in various imaging applications.
    • Effective data acquisition and control are essential for accurate detector performance.
    • Image lag can degrade the quality of data obtained from vidicon systems.

    Purpose of the Study:

    • To interface a vidicon detector with a laboratory minicomputer.
    • To characterize the vidicon's response to different illumination types and levels.
    • To compare methods for mitigating vidicon image lag.

    Main Methods:

    • Interfacing a vidicon detector with a minicomputer for scan control and data accumulation.
    • Conducting detailed characterization of vidicon response under continuous and pulsed illumination.

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    Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates

    Published on: July 9, 2020

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    Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
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  • Evaluating and comparing various techniques to eliminate detector lag.

    • Main Results:

    • Successful integration of the vidicon detector with the minicomputer system.
    • Comprehensive data on vidicon performance across a range of light intensities.
    • Identification of effective strategies for lag reduction in vidicon imaging.

    Conclusions:

    • The minicomputer interface enhances control and data acquisition for vidicon detectors.
    • Understanding vidicon response characteristics is key to optimizing imaging performance.
    • Effective lag elimination methods are crucial for high-quality vidicon-based imaging systems.