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Updated: Jun 13, 2026

Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes
Published on: October 20, 2023
Analytical and Functional Aspects of Antibody Sialylation
Johannes Stadlmann1, Martin Pabst, Friedrich Altmann
1Department of Chemistry, University of Natural Resources and Applied Life Sciences (BOKU), Muthgasse 18, 1190, Vienna, Austria.
Materials And Methods:
This review focuses on the role of antibody sialylation and methods for its quantitation. The recent attribution of the anti-inflammatory activity of IgG to the sialylation of its glycans in the Fc region has raised interest in the fine structure and analysis of the glycans. The anti-inflammatory fraction of intravenous IgG could be isolated with the Sambucus nigra lectin. Experimental strategies for the assessment of antibody sialylation are discussed.
Results:
Thorough analysis of the lectin-binding fraction revealed that the antibody Fc region only binds to S. nigra lectin when two sialic acids are present, whereas for other glycoprotein ligands, one sialic acid appears sufficient.
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