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Development and validation of an immunoluminometric assay (ILMA) for fetuin-A-glycoprotein
Amelie Bäumer1, Heinrich Schmidt-Gayk, Michael Aulmann
1Department of Operative Dentistry and Periodontology, University Hospital, Ruprecht-Karls-University Heidelberg, Heidelberg, Germany.
Background:
To identify dialysis patients with low fetuin-A levels, a sensitive immunoluminometric assay (ILMA) was developed.
Methods:
For the two-site ILMA, one monoclonal antibody was coated to polystyrene beads and one polyclonal antibody was labelled with acridinium ester.
Results:
The lower detection threshold was 0.013 g/L, with the normal range 0.20-0.87 g/L (arithmetic mean 0.437 +/- 0.118 g/L). Serum fetuin-A levels in the dialysis patients were significantly lower (arithmetic mean: 0.352 +/- 0.099 g/L, p < 0.0001).
Conclusion:
This ILMA has been proved to be a reliable method for the determination of serum fetuin-A concentrations in dialysis patients.
