Related Experiment Video
Updated: Jun 13, 2026
12:01
Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein
Published on: May 30, 2015
ELISA for RANKL-OPG complex in mouse sera.
1Department of Medicinal Biochemistry, School of Pharmacy, Aichi-Gakuin University, Nagoya, Japan. makio@dpc.aichi-gakuin.ac.jp
Journal of Immunoassay & Immunochemistry
|April 15, 2010
Summary
A new assay detects the mouse RANKL-OPG complex in serum. This complex is present in OPG+/- mice but absent in OPG-/- and wild-type mice.
Area of Science:
- Biochemistry
- Immunology
- Mouse models
Background:
- Receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) are key regulators of bone metabolism.
- Dysregulation of the RANKL/OPG system is implicated in various bone diseases.
Purpose of the Study:
- To develop a sensitive assay for quantifying the mouse RANKL-OPG complex.
- To investigate the presence of the RANKL-OPG complex in different mouse models.
Main Methods:
- Development of a sandwich enzyme-linked immunosorbent assay (ELISA) using a polyclonal antibody against mouse soluble RANKL and peroxidase-labeled mouse OPG IgG.
- Quantification of the RANKL-OPG complex with a detection limit of 1 pmol/L.
Main Results:
- The developed ELISA is sensitive and capable of quantifying the mouse RANKL-OPG complex.
- The RANKL-OPG complex was constitutively detected in the serum of OPG+/- mice.
- The RANKL-OPG complex was not detected in the serum of OPG-/- or wild-type C57BL/6 J mice.
Conclusions:
- A novel and sensitive ELISA for the mouse RANKL-OPG complex has been successfully developed.
- The findings suggest that the RANKL-OPG complex is specifically present in OPG+/- mice, indicating a potential role in their physiology.
