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Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems

Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems

Published on: August 17, 2022

DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA.

S Tabasum¹, F A Khan, S Nawaz

¹College of Agriculture, University of Sargodha, Sargodha, Pakistan.

Genetics and Molecular Research : GMR

|April 15, 2010

Summary

DNA profiling of 40 sugarcane varieties using RAPD markers revealed high genetic diversity. This research aids breeders in understanding sugarcane genetic relationships for improved crop development and conservation efforts.

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Area of Science:

Plant Genetics
Molecular Biology
Agricultural Science

Background:

Sugarcane (Saccharum officinarum and S. barberi) genetic diversity is crucial for breeding programs.
Understanding genetic relationships aids in crop improvement and germplasm conservation.

Purpose of the Study:

To construct DNA profiles for 40 sugarcane genotypes using RAPD markers.
To assess genetic distances and relationships among S. officinarum and S. barberi genotypes.
To evaluate the utility of RAPD markers for sugarcane genetic characterization.

Main Methods:

Random Amplified Polymorphic DNA (RAPD) markers were employed to analyze 40 sugarcane genotypes.
DNA profiling generated a total of 238 alleles across 30 RAPD primers, with an average of 7.93 alleles per primer.
Genetic distances were calculated using POPGENE software, and a dendrogram was constructed to visualize genetic relationships.

Main Results:

A high level of polymorphism was detected, with multiple alleles per RAPD marker.
Sugarcane genotypes clustered into two main groups, with cultivated species forming clusters alongside S. barberi genotypes.
Genetic distances between genotypes ranged from 20.29% to 64.66%.

Conclusions:

RAPD markers effectively revealed significant genetic diversity within the studied sugarcane collection.
The findings support the use of RAPD fingerprints for evaluating breeding methods and characterizing genetic pedigrees.
This genetic information is valuable for the conservation and utilization of sugarcane genetic resources.