Related Experiment Videos
Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus
M Ligozzi1, G M Rossolini, E A Tonin
1Institute of Microbiology, University of Verona, Italy.
Antimicrobial Agents and Chemotherapy
|March 1, 1991
Abstract:
A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.