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Related Experiment Video

Updated: Jun 13, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

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Published on: April 1, 2022

PDGFs regulate tooth germ proliferation and ameloblast differentiation.

Nan Wu¹, Tsutomu Iwamoto, Yu Sugawara

¹Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.

Archives of Oral Biology

|April 16, 2010

Summary

Platelet-derived growth factors (PDGFs) influence tooth development by regulating size, shape, and ameloblast differentiation. PDGF-AA specifically accelerates cusp formation and promotes cell differentiation during odontogenesis.

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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Area of Science:

Dental Biology
Developmental Biology
Cell Signaling

Background:

Tooth development (odontogenesis) is a complex process involving intricate signaling pathways.
Platelet-derived growth factors (PDGFs) are key signaling molecules implicated in various developmental processes.

Purpose of the Study:

To investigate the role of PDGFs in tooth development.
To understand the mechanisms of PDGF signaling interactions during odontogenesis.

Main Methods:

Utilized ex vivo tooth germ organ culture and dental cell lines (SF2, mDP).
Employed a tyrosine kinase inhibitor (AG17) to block PDGF receptor signaling.
Analyzed gene and protein expression using RT-PCR, immunohistochemistry, and BrdU assays.

Main Results:

PDGF ligands (PDGF-A, PDGF-B) and receptors (PDGFRalpha, PDGFRbeta) are expressed during early tooth development.
PDGF-AA accelerated cusp formation, while AG17 inhibited tooth germ growth and cusp formation.
PDGF-BB enhanced cell proliferation, and PDGF-AA modulated Fgf2 and ameloblastin expression.

Conclusions:

PDGFs play a significant role in initial tooth development.
PDGF signaling regulates tooth size, shape, and ameloblast differentiation.