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Chemical-Induced Skin Carcinogenesis Model Using Dimethylbenz[a]Anthracene and 12-O-Tetradecanoyl Phorbol-13-Acetate (DMBA-TPA)
Published on: December 19, 2019
Calcium glucarate inhibits DMBA-induced oral carcinogenesis in the hamster: histomorphometric evaluation
Carlo Lajolo1, Alessandro Sgambato, Eugenio Maiorano
1DDS, School of Dentistry, Largo A. Gemelli, 8; 00168 Rome, Italy. carlo.lajolo@fastwebnet.it
Background:
Calcium glucarate (CGT) is a promising chemopreventive agent. This study evaluated the in vivo efficacy of CGT in preventing 7,12-dimethylbenz(alpha) anthracene (DMBA)-induced oral carcinogenesis in the hamster. Matherials and Methods: Seventy-six Syrian hamsters were used, divided into four groups: group 1, untreated animals; 2, CGT controls; 3, DMBA-treated; 4, DMBA- and CGT-treated. Hamsters were painted three times weekly with 0.5% solution of DMBA and were fed a diet supplemented with CGT (64 mmol/kg, 2%). Animals were sacrificed at week 9 and 12 and pathology and histomorphometric analyses were performed.
Results:
At week 9, four dysplastic lesions and six carcinomas were identified in group 3 while only three dysplasias and five carcinomas were detected in group 4. At week 12, five animals of group 3 displayed a dysplasia, which was only detected in one animal of group 4. Squamous carcinomas were identified in all animals of both group 3 and 4. However, in group 3 four of the animals displayed multifocal lesions and carcinomas displayed histological features indicative of increased aggressiveness.
Conclusion:
The results obtained suggest that CGT can exert an inhibitory effect on oral carcinogenesis in tha hamster and that further studies are warranted to evaluate its potential use as a chemopreventive agent in humans.
Insights
Calcium glucarate (CGT) shows potential in preventing oral cancer. This study found CGT reduced the severity and aggressiveness of oral tumors induced by DMBA in hamsters.
Area of Science:
- Oncology
- Chemoprevention
- Oral Carcinogenesis
Background:
- Calcium glucarate (CGT) is recognized as a potential chemopreventive agent.
- Oral carcinogenesis is a significant health concern globally.
- Investigating natural compounds for cancer prevention is crucial.
Purpose of the Study:
- To evaluate the efficacy of Calcium glucarate (CGT) in preventing 7,12-dimethylbenz(alpha)anthracene (DMBA)-induced oral carcinogenesis.
- To assess the impact of CGT on the development and progression of oral tumors in a hamster model.
- To determine if CGT can inhibit DMBA-induced oral cancer development.
Main Methods:
- Utilized a hamster model for oral carcinogenesis, induced by 7,12-dimethylbenz(alpha)anthracene (DMBA).
- Administered Calcium glucarate (CGT) orally to experimental groups.
- Performed pathological and histomorphometric analyses at 9 and 12 weeks post-induction.
Main Results:
- DMBA-treated hamsters (Group 3) showed more dysplastic lesions and aggressive carcinomas compared to DMBA- and CGT-treated hamsters (Group 4) at week 9.
- At week 12, fewer dysplastic lesions were observed in the CGT-treated group (Group 4).
- While squamous carcinomas were present in both DMBA-treated groups, Group 3 exhibited multifocal lesions and increased aggressiveness.
Conclusions:
- Calcium glucarate (CGT) demonstrates an inhibitory effect on oral carcinogenesis in the hamster model.
- CGT may reduce the progression and aggressiveness of DMBA-induced oral tumors.
- Further research is recommended to explore CGT's potential as a human chemopreventive agent for oral cancer.
