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[RAPD analysis for genetic diversity of medicinal plant Coptis omeiensis]
Chunping Zhang1, Ping He, Junxing He
1School of Life Sciences, Southwest University, Key Laboratory (Ministry of Education) of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology and Resources Research for Three Gorges Reservoir Region, Chongqing 400715, China. chunpingzhang520@163.com
Objective:
To discuss the genetic diversity of Coptis omeiensis.
Method:
The genetic diversity of 110 individuals from 10 populations was analyzed by RAPD.
Result:
14 primers were selected to produce highly reproducible RAPD bands. Among 132 amplified bands, 98 showed polymorphism, the percentage of polymorphic bands reached to 74.24%. Nei's gene diversity index (H) was 0.2863, Shannon's information index (I) was 0.3624, G(st) was 0.2305. The genetic distance coefficient and the similarity were 0.1931-0.5245 and 0.5016-0.8843, respectively.
Conclusion:
There exists a held high genetic diversity in C. omeiensis and the majority of genetic variation occurs in the populations. By cluster analysis, the geographical distribution is very obvious. The RAPD marker can be used for the analysis of the genetic diversity and genetic variation of C. omeiensis.
