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[HPLC fingerprint of Codonopsis tangshen from different habitats]
Rui Peng1, Peng Ma, Longyun Li
1Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
Objective:
To establish a sensitive and specific HPLC method for quality control of Codonopsis tangshen.
Method:
The samples were determined with the dereloped method. By the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004A), mean chromatogram was generated as the representative standard fingerprint and the similarity of each chromatogram against the mean chromatogram was also calculated. Samples was clustered using principal component analysis (PCA) based on the ratio of characteristic peaks and standard peak. The chromatographic fingerprinting of C. tangshen, showing 7 characteristic peaks, was established from 29 habitats of C. tangshen.
Result:
The similarity of the chromatographic fingerprints from the 29 samples was over 0.8 and 23 samples were classified into one group based on hierarchical cluster, which showed the quality of C. tangshen from different habitats was in good consistency.
Conclusion:
The method can be applied for quality assessment of C. tangshen.
