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[Simultaneous determination of three active compounds in root barks of Dictamnus dasycarpus by RP-HPLC]
Xiaojuan Yang1, Yanfang Liu, Zhong Bao
1State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
Objective:
To develop a RP-HPLC method for simultaneous determination of three active compounds, dictamnine, obacunone and fraxinellone in root bark of Dictamnus dasycarpus and supply a reference for the establishment of the quality standard of D. dasycarpus.
Method:
A Kromasil C18 column was used with methanol-water (60:40) as the mobile phase, at the flow rate of 1 mL x min(-1). 236 nm was selected as the detected wavelength.
Result:
The determined three compounds were well separated with a linear range of 0.0021-0.1060, 0.0201-0.9200 and 0.0102-1.020 g x L(-1), respectively. The recoveries of them were 100.5%, 99.2% and 100.2%, respectively.
Conclusion:
This method is simple, rapid and accurate, particularly suitable for the quality control of D. dasycarpus.