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Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...

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Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
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Dynamic angular segregation of vesicles in electrohydrodynamic flows.

William D Ristenpart1, Olivier Vincent, Sigolene Lecuyer

  • 1Department of Chemical Engineering & Materials Science, University of California at Davis, Davis, California 95616, USA. wdristenpart@ucdavis.edu

Langmuir : the ACS Journal of Surfaces and Colloids
|April 17, 2010
PubMed
Summary
This summary is machine-generated.

Small vesicles orbiting larger ones in an electric field exhibit dynamic angular segregation. This behavior, driven by induced dipolar interactions, forms distinct vesicle bands in a toroidal flow.

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Area of Science:

  • Physics, soft matter
  • Biophysics

Background:

  • Electric fields induce aggregation in vesicle suspensions.
  • Toroidal electrohydrodynamic flow fields can trap particles.

Purpose of the Study:

  • Investigate dynamic angular segregation of small vesicles orbiting larger ones.
  • Model the behavior of vesicles in electrohydrodynamic flow.

Main Methods:

  • Applied low-frequency electric fields to vesicle suspensions.
  • Observed vesicle behavior in a toroidal electrohydrodynamic flow.
  • Developed a model based on rotating point dipoles.

Main Results:

  • Small vesicles (<10 microm) orbit larger vesicles (>20 microm).
  • Observed dynamic angular segregation of orbiting vesicles into distinct bands.
  • Model accurately predicted diverse vesicle trajectories.

Conclusions:

  • Angular segregation is explained by induced dipolar interactions.
  • The point dipole model successfully captures vesicle dynamics in cellular flow.
  • This behavior offers insights into particle self-organization in complex fluids.