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Related Concept Videos

Mass Spectrometry: Long-Chain Alkane Fragmentation01:18

Mass Spectrometry: Long-Chain Alkane Fragmentation

The molecular ions of linear alkanes prefer to fragment at the carbon-carbon bond away from the end of the chain since the cleavage of an inner bond creates a stable carbocation and a stable radical. Consequently, the mass signals of linear alkanes feature intense peaks in the middle of the mass-to-charge ratio plot with weaker peaks on either end. The fragmentation of each carbon-carbon bond with the release of a methyl group in each splitting leads to prominent peaks in the mass spectra...
Mass Spectrometry: Branched Alkane Fragmentation01:29

Mass Spectrometry: Branched Alkane Fragmentation

This lesson delves into the mass spectrometry of branched alkane fragmentation. Branched alkanes possess secondary or tertiary carbon atoms, which generate relatively stable carbocations if the cleavage occurs at the branching point. The high stability of carbocations drives the instant fragmentation of branched alkanes. Accordingly, the branched alkane's molecular ion peak is very weak or invisible in the mass spectra, especially in comparison to a linear alkane.
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

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Related Experiment Video

Updated: Jun 13, 2026

Chemical Analysis of Water-accommodated Fractions of Crude Oil Spills Using TIMS-FT-ICR MS
08:17

Chemical Analysis of Water-accommodated Fractions of Crude Oil Spills Using TIMS-FT-ICR MS

Published on: March 3, 2017

Petroleomics by EASI(+/-) FT-ICR MS.

Yuri E Corilo1, Boniek G Vaz, Rosineide C Simas

  • 1ThoMSon Mass Spectrometry Laboratory, University of Campinas-UNICAMP, Institute of Chemistry, 13083-970 Campinas, SP, Brazil.

Analytical Chemistry
|April 17, 2010
PubMed
Summary
This summary is machine-generated.

Easy ambient sonic-spray ionization mass spectrometry (EASI) analyzes crude oil components efficiently. This voltage-free technique requires no sample preparation, offering rapid and reliable petroleomic analysis.

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Last Updated: Jun 13, 2026

Chemical Analysis of Water-accommodated Fractions of Crude Oil Spills Using TIMS-FT-ICR MS
08:17

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Published on: March 3, 2017

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Published on: March 18, 2022

On-line Analysis of Nitrogen Containing Compounds in Complex Hydrocarbon Matrixes
07:49

On-line Analysis of Nitrogen Containing Compounds in Complex Hydrocarbon Matrixes

Published on: August 5, 2016

Area of Science:

  • Analytical Chemistry
  • Petrochemistry
  • Mass Spectrometry

Background:

  • Crude oil analysis requires advanced techniques for detailed compositional understanding.
  • Existing ambient ionization methods may involve complex sample preparation or voltage-dependent artifacts.

Purpose of the Study:

  • To evaluate the efficacy of easy ambient sonic-spray ionization mass spectrometry (EASI) for crude oil analysis.
  • To demonstrate EASI as a rapid, voltage-free, and workup-free alternative for petroleomic studies.

Main Methods:

  • Application of EASI(+/-) to crude oil samples on an inert surface.
  • Analysis of generated gaseous ions using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS).
  • Comparison of EASI(+/-) FT-ICR MS results with conventional Electrospray Ionization (ESI) methods.

Main Results:

  • EASI(+/-) efficiently desorbed and ionized a vast number of polar components from crude oil.
  • Approximately 6000 acidic and basic components were identified.
  • EASI(+/-) FT-ICR MS provided compositional information comparable to ESI FT-ICR MS in speed and quality.
  • The technique eliminated the need for sample workup, heating, and voltage, preventing contamination and redox side reactions.

Conclusions:

  • EASI(+/-) FT-ICR MS is a powerful, rapid, and robust technique for comprehensive petroleomic analysis of crude oils.
  • Its voltage-free and workup-free nature simplifies analysis and enhances reliability.
  • EASI offers a significant advancement for open-atmosphere, direct analysis of complex hydrocarbon mixtures.