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Updated: Jun 13, 2026
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Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
Published on: August 9, 2012
Remodeling of buccal mucosa by bladder microenvironment
Mujun Lu1, Guangdong Zhou, Wei Liu
1Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China.
Urology
|April 17, 2010
Summary
Oral mucosa can transform into bladder urothelium when transplanted into the bladder. This suggests oral keratinocytes are a promising cell source for bladder reconstruction.
Area of Science:
- Regenerative Medicine
- Tissue Engineering
- Urology
Background:
- The optimal cell source for bladder reconstruction remains unclear.
- Investigating alternative tissues for bladder mucosa repair is crucial.
Purpose of the Study:
- To explore the feasibility of oral mucosa transforming into bladder urothelium.
- To assess the role of the bladder microenvironment in this transformation.
Main Methods:
- Porcine oral mucosa grafts were transplanted into autologous bladder defects.
- Immunohistochemistry and RT-PCR were used to analyze marker expression at 3, 6, and 12 months.
- Specific markers included cytokeratin 14 (CK 14) for oral epithelium and uroplakin II (UPII) for urothelium.
Main Results:
- Oral mucosa grafts maintained their phenotype for 3 months, expressing CK 14 and not UPII.
- After 6 months, grafts began expressing UPII at protein and mRNA levels, indicating transformation.
- This urothelial phenotype persisted at 12 months, while CK 14 expression remained positive.
Conclusions:
- Oral keratinocytes demonstrate transdifferentiation potential towards urothelial cells.
- The bladder microenvironment plays a key role in remodeling oral mucosa.
- Oral keratinocytes represent a viable cell source for bladder mucosa reconstruction.
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