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Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii
Published on: April 16, 2016
Single-spore elemental analyses indicate that dipicolinic acid-deficient Bacillus subtilis spores fail to accumulate
Paul E Hintze1, Wayne L Nicholson
1NASA Corrosion Technology Laboratory, Kennedy Space Center, Gainesville, FL 32899, USA.
Abstract:
Dipicolinic acid (pyridine-2,6-carboxylic acid; DPA) is a major component of bacterial spores and has been shown to be an important determinant of spore resistance. In the core of dormant Bacillus subtilis spores, DPA is associated with divalent calcium in a 1:1 chelate (Ca-DPA). Spores excrete Ca-DPA during germination, but it is unknown whether Ca and DPA are imported separately or together into the developing spore. Elemental analysis by scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDS) of wild-type spores and mutant spores lacking the ability to synthesize DPA showed that DPA-less spores also lacked calcium, suggesting that the two compounds may be co-imported.
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