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Related Concept Videos

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

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Related Experiment Video

Updated: Jun 13, 2026

Operation of a Benchtop Bioreactor
12:54

Operation of a Benchtop Bioreactor

Published on: September 12, 2013

Bioreactors and bioseparation.

Siliang Zhang1, Xuejun Cao, Ju Chu

  • 1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China, siliangz@ecust.edu.cn.

Advances in Biochemical Engineering/Biotechnology
|April 17, 2010
PubMed
Summary
This summary is machine-generated.

Chinese bioreactor and bioproduct separation technologies are advancing rapidly. Multiscale analysis and computational fluid dynamics optimize bioprocesses, improving production efficiency for biotechnological products.

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Last Updated: Jun 13, 2026

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Process Optimization

Background:

  • Cell cultivation technology is crucial for biotechnological products.
  • Bioreactors and bioproduct separation are key industrial platforms.
  • China's advancements in these areas are significant.

Purpose of the Study:

  • To systematically discuss bioreactor techniques in China.
  • To elucidate bioreactor processes and optimization principles.
  • To introduce bioproduct separation technologies.

Main Methods:

  • Multiscale analysis (genetic, cellular, bioreactor levels) of bioprocesses.
  • Metabolic flux analysis and association analysis of multiscale parameters.
  • Computational fluid dynamics (CFD) for flow field studies in bioreactor design.
  • Review of progress in bioseparation engineering techniques.

Main Results:

  • Bioreactors manufactured in China are widely used in research and production.
  • Optimization approaches have significantly improved production efficiency.
  • Progress has been made in chromatography media, ATPS, affinity precipitation, and other separation techniques.

Conclusions:

  • Multiscale consideration and systems biology are vital for future bioprocess development.
  • Development of microbioreactors and large-scale animal cell bioreactors is significant.
  • Bioseparation engineering in China has achieved considerable progress.