Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...
Poliomyelitis01:17

Poliomyelitis

Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Coronavirus01:29

Coronavirus

Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association Between Reported Homelessness and Inpatient Resource Use for Medicare Beneficiaries.

Journal of health care for the poor and underserved·2026
Same author

Genetic screening for CSF1R variants in patients with dementia, parkinsonism, and multiple sclerosis.

Neurologia i neurochirurgia polska·2026
Same author

Under-Documentation of Z-Codes in Hospitalizations of Homeless Shelter Users in New York City.

Health services research·2026
Same author

Clinical and neuropathological analysis of the most common CSF1R p.Ile794Thr mutation.

Neurologia i neurochirurgia polska·2026
Same author

Pharmacological inhibition of PMS2 induces MMR deficiency and response to immune checkpoint blockade.

Cancer discovery·2026
Same author

Tulane virus protease as a structural surrogate for inhibitor screening of human norovirus proteases.

Journal of virology·2026
Same journal

Resolution Failure in Periodontal Diseases: Dysregulated Pro-resolving Mechanisms in Chronic Inflammation and Tissue Breakdown.

Current topics in microbiology and immunology·2026
Same journal

Correction to: Resolution of Skeletal Muscle Inflammation: Role of Specialized Pro-resolving Lipid Mediators in the Recovery from Exercise, Injury, and Disease.

Current topics in microbiology and immunology·2026
Same journal

Resolution Biology in Soft Tissue Joint Disease.

Current topics in microbiology and immunology·2026
Same journal

A 25+ Year Journey on Yeast-Regulated Cell Death Research.

Current topics in microbiology and immunology·2026
Same journal

Adoptive T-Cell Immunotherapy.

Current topics in microbiology and immunology·2026
Same journal

Resolution Pharmacology Targeting the Melanocortin System.

Current topics in microbiology and immunology·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Rotavirus cell entry.

Matthew Baker1, B V Venkataram Prasad

  • 1National Center for Macromolecular Imaging, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.

Current Topics in Microbiology and Immunology
|April 17, 2010
PubMed
Summary
This summary is machine-generated.

Rotaviruses, a common cause of severe gastroenteritis in children, enter cells through a complex process involving specific receptors and unique structural changes. Understanding this viral cell entry mechanism is key to developing effective treatments.

More Related Videos

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators
08:01

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators

Published on: January 19, 2024

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

Related Experiment Videos

Last Updated: Jun 13, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators
08:01

Live Calcium Imaging of Virus-Infected Human Intestinal Organoid Monolayers Using Genetically Encoded Calcium Indicators

Published on: January 19, 2024

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Rotaviruses are a leading cause of severe gastroenteritis in young children worldwide.
  • The precise mechanisms of rotavirus cell entry remain incompletely understood despite extensive research.

Purpose of the Study:

  • To elucidate the complex cellular and molecular events involved in rotavirus cell entry.
  • To investigate the role of viral components and host cell factors in viral internalization.

Main Methods:

  • Analysis of viral attachment and entry pathways.
  • Characterization of viral protein interactions with host cell receptors.
  • Investigation of cellular mechanisms, including lipid rafts and endocytosis pathways.

Main Results:

  • Rotavirus cell entry involves proteolytic priming and attachment to sialic acid receptors.
  • Postattachment receptors, including integrins and Hsc70, localize to lipid rafts.
  • Viral internalization appears independent of clathrin- and caveola-mediated endocytosis.
  • The VP4 spikes undergo significant structural changes during cell entry.

Conclusions:

  • Rotavirus cell entry is a complex, coordinated process involving specific host-pathogen interactions.
  • The internalization pathway utilizes unique cellular mechanisms distinct from canonical endocytosis.
  • Further research is needed to fully understand the structural dynamics and molecular details of rotavirus entry.