Morphology of neuropeptide CNP2 modulation of heart activity in terrestrial snail

A family of neuropeptides called Command Neuron Peptides (CNPs) was described ten years ago as the protein products of the gene HCS2, specifically expressed in the identified interneurons of the nervous system of terrestrial snail (Helix lucorum L. and H. pomatia L.). Recently, the CNP-like peptides have been detected by immunochemistry and immunoblotting in nervous systems of representatives of different invertebrate phyla (Mollusca, Annelida, and Insecta). Still, the function of these peptides remains largely unknown. In Helix it is shown that CNPs: modulate the electrical activity of unidentified central neurons, modulate the pneumostome motoneurons, stimulate neural cones growth in neural cultures. Here, we describe for the first time the CNPs-immunoreactive neural fibers in walls of both auricle and ventricle of the snail heart. We show that application of the synthetic neuropeptide CNP2 (DYPRLamide) in perfusion saline affects heart rate and magnitude of beats in isolated snail heart. The results suggest that in Helix the Command Neuron Peptides could participate in neural modulation of cardiovascular system.