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Updated: Jun 13, 2026

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Published on: January 26, 2017

The tandem affinity purification method: an efficient system for protein complex purification and protein interaction

Xiaoli Xu¹, Yuan Song, Yuhua Li

¹Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.

Protein Expression and Purification

|April 20, 2010

Summary

The tandem affinity purification (TAP) method enables protein complex analysis across various organisms. This review details TAP method modifications, applications, and future prospects for identifying protein interactions.

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Area of Science:

Molecular Biology
Biochemistry
Proteomics

Background:

Understanding protein interactions is crucial for elucidating cellular functions and molecular mechanisms.
The tandem affinity purification (TAP) method was initially developed for yeast to purify protein complexes and identify protein-protein interactions.

Purpose of the Study:

To review the applications of the TAP method in diverse organisms.
To discuss modifications and advancements in TAP tag technology.
To explore the limitations and future potential of TAP for protein complex analysis.

Main Methods:

Literature review of the TAP method and its variations.
Analysis of TAP method applications across different biological systems (yeast, mammalian, plant, bacteria).
Examination of tag modifications and their impact on purification efficiency.

Main Results:

The TAP method has been successfully adapted for protein complex analysis in various organisms beyond yeast.
Numerous modifications to the original TAP tag have enhanced its applicability and efficiency.
The review covers disadvantages, recent developments, and future directions for the TAP technique.

Conclusions:

The TAP method is a versatile and adaptable technique for protein complex purification and interaction studies.
Ongoing modifications and developments continue to expand the utility of TAP in biological research.
The future prospects of TAP involve further refinement for high-throughput analysis and complex interactome mapping.