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A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
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A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

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Comparative study of three porcine Salmonella ELISAs

M-C Poulin1, M Donadeu, E K Mackay

  • 1PIC, 2 Kingston Business Park, Kingston Bagpuize, Oxfordshire OX13 5FE. marie-claude.poulin@hotmail.co.uk

The Veterinary Record
|April 20, 2010
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.