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Updated: Jun 13, 2026
Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus
Published on: January 23, 2018
Can osteochondral grafting be augmented with microfracture in an extended-size lesion of articular cartilage?
John G Lane1, Robert M Healey, Albert C Chen
1Department of Orthopaedic Surgery, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093-0863, USA.
Background:
Both microfracture and osteochondral autografting procedures have been useful in treating osteochondral lesions.
Hypothesis:
Combining microfracture and osteochondral autografting procedures can extend the size of lesions that can be treated with either technique.
Study Design:
Descriptive laboratory study.
Methods:
Eight adult goats underwent osteochondral autograft transfer of a 4.5-mm femoral trochlea plug into an 8-mm full-thickness chondral defect. Microfracture was performed in the gap region surrounding the autograft. The animals were allowed normal activity until the end of the experiment at 6 months. At harvest, the knees were assessed grossly, and then evaluation was performed by histology, histomorphometry, biochemistry, and biomechanics.
Results:
The osteochondral plugs healed well, with integration of the bone and preservation of the chondral cap. The chondral gap between the host site articular cartilage and the transferred plug had decreased from 3 mm at implant to less than 0.1 mm. Histologic analysis demonstrated regions of variable cartilage repair, with integration of the cartilage layer at some sites but incomplete healing at others. Histomorphometry demonstrated filling of the chondral gap to 75% to 85% of the normal volume. Biochemical analysis revealed greater than 90% type II collagen at most sites, with some areas containing 80% type II collagen. Biomechanical indentation testing indicated that the repaired area had variable thickness and stiffness, with a trend of increased stiffness in the bulk graft and decreased softness at the proximal microfracture interface site.
Conclusion:
The performance of a combined microfracture and osteochondral autograft transfer procedure to resurface a large chondral defect appears promising.
Clinical Relevance:
This combined technique shows promise for treatment of large chondral defects with a single operative procedure with autogenous tissue that is safe and potentially would have a shorter period of rehabilitation, similar to that of osteochondral transfers and microfracture, in a cost-effective setting.
