Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrophoretic separation of 5'-nucleotidase multiple forms.

F J Novo1, J C Tutor

  • 1Laboratorio Central, Hospital General de Galicia, Santiago de Compostela, Spain.

Clinical Biochemistry
|April 1, 1991
PubMed
Summary

This study introduces cellulose acetate electrophoresis for separating 5'-nucleotidase forms. In hepatobiliary diseases, the alpha 1 isoform of 5'-nucleotidase increases, correlating with alkaline phosphatase changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bioinformatic analyses of CALR mutations in myeloproliferative neoplasms support a role in signaling.

Leukemia·2014
Same author

[Valproic acid serum through concentrations estimated from 12 hours post-dose concentrations in patients treated with Depakine® Crono].

Farmacia hospitalaria : organo oficial de expresion cientifica de la Sociedad Espanola de Farmacia Hospitalaria·2013
Same author

Diagnostic accuracy of a hyperbolic model in predicting digoxin concentrations based on glomerular filtration rates.

Nefrologia : publicacion oficial de la Sociedad Espanola Nefrologia·2011
Same author

LNK can also be mutated outside PH and SH2 domains in myeloproliferative neoplasms with and without V617FJAK2 mutation.

Leukemia research·2011
Same author

Two sampling time profiles for the abbreviated estimation of mycophenolic acid area under the curve in adult renal transplant recipients treated with mycophenolate mofetil and concomitant tacrolimus.

Journal of clinical pharmacy and therapeutics·2009
Same author

[Negative results related to drugs required in hospitalisation].

Farmacia hospitalaria : organo oficial de expresion cientifica de la Sociedad Espanola de Farmacia Hospitalaria·2009

Area of Science:

  • Biochemistry
  • Enzymology
  • Clinical Chemistry

Background:

  • 5 -nucleotidase (5 -NT) is an enzyme crucial in nucleotide metabolism.
  • Multiple isoforms of 5 -NT exist, with varying roles and clinical significance.
  • Understanding 5 -NT isoform behavior is important for diagnosing liver diseases.

Purpose of the Study:

  • To develop and validate a cellulose acetate electrophoresis method for separating 5 -nucleotidase isoforms.
  • To investigate the impact of various biochemical treatments on 5 -NT and alkaline phosphatase (ALP) electrophoretic patterns.
  • To analyze alterations in 5 -NT isoforms in patients with hepatobiliary diseases.

Main Methods:

  • Cellulose acetate electrophoresis was employed to separate 5 -NT isoforms.
  • Enzyme activity was analyzed after various treatments including lectins, enzymes, detergents, and precipitation methods.

Related Experiment Videos

  • Electrophoretic patterns of 5 -NT and ALP were compared between healthy controls and patients with hepatobiliary diseases.

    • Main Results:

    • Healthy individuals exhibited three main 5 -NT fractions: alpha 1, alpha 2, and beta, with beta being predominant.
    • Hepatobiliary diseases showed a correlation between high molecular weight ALP and an elevated Total/beta 5 -NT ratio.
    • The increase in total 5 -NT in these patients was primarily attributed to the alpha 1 isoform.

    Conclusions:

    • Cellulose acetate electrophoresis is an effective method for differentiating 5 -NT isoforms.
    • The alpha 1 5 -NT isoform appears to be significantly involved in the pathophysiology of hepatobiliary diseases.
    • Further research into the specific roles and regulation of 5 -NT isoforms is warranted.