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Related Concept Videos

Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
Ion Channels01:19

Ion Channels

The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...

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Related Experiment Video

Updated: Jun 13, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

Mechanosensitive gating of CFTR.

Wei Kevin Zhang1, Dong Wang, Yuanyuan Duan

  • 1Nano Science and Technology Program, The Hong Kong University of Science and Technology, Hong Kong SAR, People's Republic of China.

Nature Cell Biology
|April 20, 2010
PubMed
Summary

The cystic fibrosis transmembrane conductance regulator (CFTR) channel is activated by membrane stretch, revealing a new role in mechanosensing. This discovery has implications for understanding ion transport and cell volume regulation in the body.

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Last Updated: Jun 13, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
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Published on: August 13, 2012

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Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
09:54

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

Area of Science:

  • Physiology
  • Molecular Biology
  • Biophysics

Background:

  • Cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel linked to cystic fibrosis.
  • CFTR functions as an intracellular ligand-gated channel and regulates other membrane transporters.

Purpose of the Study:

  • To investigate the role of membrane stretch in activating CFTR.
  • To determine if CFTR possesses mechanosensitive properties.

Main Methods:

  • Single-channel, cellular, and tissue-level electrophysiological recordings were used.
  • Negative pressures were applied to induce membrane stretch.
  • Chloride transport was measured in Calu-3 human airway epithelial cells and mouse intestinal tissues.

Main Results:

  • CFTR was robustly activated by membrane stretch at pressures as low as 5 mmHg.
  • Stretch increased the NPo (open channel probability and number) and unitary conductance of CFTR.
  • Stretch-mediated activation of CFTR resulted in chloride transport.

Conclusions:

  • CFTR exhibits intrinsic mechanosensitivity, functioning as a mechanosensitive anion channel.
  • This mechanosensitivity has significant physiological implications for epithelial ion transport and cell volume regulation.
  • CFTR's role extends beyond anion channel function to include mechanosensing in dynamic cellular environments.