Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Clinical Significance of Antibiotic Resistance

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Mechanism of Antibiotic Resistance in MRSA

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quantifying patient- and hospital-level antimicrobial resistance dynamics in <i>Staphylococcus aureus</i> from routinely collected data.

Journal of medical microbiology·2023

Same author

Markers of epidemiological success of methicillin-resistant Staphylococcus aureus isolates in European populations.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2023

Same author

Quantifying patient- and hospital-level antimicrobial resistance dynamics in <i>Staphylococcus aureus</i> from routinely collected data.

medRxiv : the preprint server for health sciences·2023

Same author

Correction for Leclerc et al., "Growth-Dependent Predation and Generalized Transduction of Antimicrobial Resistance by Bacteriophage".

mSystems·2022

Same author

Modelling the synergistic effect of bacteriophage and antibiotics on bacteria: Killers and drivers of resistance evolution.

PLoS computational biology·2022

Same author

Growth-Dependent Predation and Generalized Transduction of Antimicrobial Resistance by Bacteriophage.

mSystems·2022

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

The RM test for determining methicillin-resistant Staphylococcus aureus lineages.

Jodi A Lindsay¹, Julia M-L Sung

¹Department Cellular & Molecular Medicine, Centre for Infection, St George's University of London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|April 20, 2010

Summary

A new PCR-based method rapidly identifies Staphylococcus aureus lineages using restriction modification (RM) systems. This cost-effective approach offers quick results for epidemiological typing and infection control.

Related Experiment Videos

Last Updated: Jun 13, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Area of Science:

Microbiology
Genetics
Epidemiology

Background:

Staphylococcus aureus exhibits independent lineage evolution, with genetic differences impacting pathogenesis.
Accurate lineage identification is crucial for epidemiological typing, infection control, and understanding strain-specific virulence.
Current methods like MLST and spa typing are effective but time-consuming and costly.

Purpose of the Study:

To develop a rapid, cost-effective, and easy-to-perform method for identifying Staphylococcus aureus lineages.
To leverage unique lineage-specific sequences for a novel diagnostic test.
To provide a scalable solution adaptable to various laboratory platforms.

Main Methods:

Development of a Polymerase Chain Reaction (PCR)-based assay targeting unique lineage-specific sequences.
Utilizing the principle of restriction modification (RM) systems, which are specific to each S. aureus lineage.
Adaptation of the PCR-R M test for potential use on commercial and real-time PCR platforms.

Main Results:

The developed PCR-based method allows for rapid identification of S. aureus lineages within hours.
The method is based on the specificity of restriction modification systems unique to each lineage.
The technique is easily performed and has the potential for broad application in clinical and research settings.

Conclusions:

A novel, rapid PCR-based restriction modification (RM) test effectively identifies Staphylococcus aureus lineages.
This method offers a significant improvement over traditional typing techniques in terms of speed and ease of use.
The RM test is a valuable tool for epidemiological surveillance, infection control, and comparative pathogenesis studies of S. aureus.