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Diversity of Protists II

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Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
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Published on: October 16, 2016

Polymorphism and divergence within the ascidian genus Ciona.

Marie L Nydam1, Richard G Harrison

  • 1Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY 14853, USA. mln32@cornell.edu

Molecular Phylogenetics and Evolution
|April 21, 2010
PubMed
Summary

Gene genealogies reveal distinct evolutionary relationships within the Ciona genus, identifying well-supported monophyletic groups for Ciona intestinalis Types A and B. This study clarifies ascidian evolutionary history and genetic diversity.

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Area of Science:

  • Marine Biology
  • Evolutionary Biology
  • Genetics

Background:

  • The genus Ciona, a model organism in developmental biology, is also noted for high heterozygosity, prompting evolutionary interest.
  • Previous research identified Ciona savignyi as an outgroup and Ciona intestinalis as having two forms (Types A and B).
  • Genealogical relationships within Ciona have been underexplored despite the genus's significance.

Purpose of the Study:

  • To investigate the genealogical relationships among Ciona species and forms.
  • To estimate levels of genetic polymorphism in natural Ciona populations.
  • To clarify the evolutionary divergence between Ciona intestinalis Types A and B and their relationship with other Ciona species.

Main Methods:

  • Phylogenetic analysis using gene genealogies of six nuclear gene loci.
  • Comparison of genetic divergence between Ciona intestinalis Types A and B.
  • Examination of the placement of Ciona roulei and a new Ciona species within the established genealogies.

Main Results:

  • Gene genealogies strongly support Ciona intestinalis Types A and B as distinct monophyletic groups.
  • Genetic divergence between Ciona intestinalis Types A and B ranges from 0.035 to 0.124.
  • Ciona roulei and a new Ciona species are nested within Type B lineages, separate from Type A.

Conclusions:

  • The study provides robust evidence for the distinct evolutionary trajectories of Ciona intestinalis Types A and B.
  • Morphological similarity masks significant genetic divergence between these ascidian forms.
  • The findings contribute to understanding ascidian evolutionary history and the genetic architecture of natural populations.