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Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...

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Related Experiment Video

Updated: Jun 13, 2026

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Free centrosomes: where do they all come from?

Vincent Archambault1, Xavier Pinson

  • 1Institut de recherche en Immunologie et en Cancérologie, Université de Montréal, QC, Canada. vincent.archambault.1@umontreal.ca

Fly
|April 21, 2010
PubMed
Summary

Free centrosomes in Drosophila embryos, though often overlooked, can reveal insights into cell cycle regulation and cytoskeletal dynamics. Investigating their occurrence offers valuable clues about molecular pathways affecting centrosome function.

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Last Updated: Jun 13, 2026

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
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Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

Area of Science:

  • Cell Biology
  • Cytoskeletal Dynamics
  • Molecular Biology

Background:

  • Centrosomes are crucial microtubule-organizing centers, typically associated with the nucleus for proper function.
  • This nuclear association is vital, especially in large cells.
  • Free centrosomes have been observed in syncytial Drosophila embryos but are often underexplored.

Purpose of the Study:

  • To explore the significance and underlying mechanisms of free centrosomes in Drosophila embryos.
  • To understand how free centrosomes can provide insights into cell cycle regulation and cytoskeletal dynamics.
  • To highlight the utility of studying free centrosomes for deciphering affected molecular pathways.

Main Methods:

  • Genetic analysis and cytological testing in Drosophila embryos.
  • Time-lapse imaging to observe centrosome behavior.
  • Perturbation studies using mutations or drug injections.

Main Results:

  • Free centrosomes can arise through various mechanisms, including mitotic spindle defects, nuclear envelope detachment, DNA damage-induced inactivation, or de novo formation.
  • Studying these free centrosomes offers a window into specific molecular pathways.

Conclusions:

  • Investigating free centrosomes in Drosophila embryos is a valuable approach to understand fundamental cell biology processes.
  • This research can illuminate mechanisms of centrosome function, cell cycle control, and cytoskeletal dynamics.
  • Understanding the origins of free centrosomes aids in interpreting cellular responses to perturbations.