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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...

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Related Experiment Video

Updated: Jun 13, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

miR-129 regulates cell proliferation by downregulating Cdk6 expression.

Junjie Wu1, Jun Qian, Chun Li

  • 1Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA, USA. jining@bu.edu

Cell Cycle (Georgetown, Tex.)
|April 21, 2010
PubMed
Summary
This summary is machine-generated.

MicroRNA-129 (miR-129) inhibits tumor cell proliferation by targeting Cdk6, a key regulator of the G1/S phase transition. Overexpression of miR-129 induces cell cycle arrest and death, highlighting its tumor-suppressive role.

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An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

Related Experiment Videos

Last Updated: Jun 13, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

Area of Science:

  • Molecular Biology
  • Oncology
  • Cell Biology

Background:

  • Reduced microRNA-129 (miR-129) expression is observed in various cancers, including lung adenocarcinoma.
  • miR-129 exhibits anti-proliferative activity in tumor cell lines, but its regulatory mechanisms remain unclear.

Purpose of the Study:

  • To investigate the role of miR-129 in regulating cell proliferation.
  • To identify the molecular targets of miR-129 involved in cell cycle control.

Main Methods:

  • Lentiviral-mediated overexpression of miR-129 in mouse lung epithelial cells (E10) and human lung adenocarcinoma cell lines.
  • Cell cycle analysis (G1 phase arrest).
  • Target validation using luciferase reporter assays and Western blotting to assess Cdk6, Erk1, Erk2, and Prkce expression.

Main Results:

  • Overexpression of miR-129 induced significant G1 phase arrest and cell death in lung epithelial and adenocarcinoma cells.
  • Cyclin-dependent kinase 6 (Cdk6) was identified as a direct target of miR-129.
  • Restoring Cdk6 expression partially rescued the cell growth arrest and cell death phenotypes.

Conclusions:

  • miR-129 functions as a tumor suppressor by inhibiting cell proliferation.
  • The primary mechanism involves the downregulation of its direct target, Cdk6, leading to G1 phase arrest.