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06:04
Murine Dermal Fibroblast Isolation by FACS
Published on: January 7, 2016
Human fibroblasts share immunosuppressive properties with bone marrow mesenchymal stem cells
Sandrine Cappellesso-Fleury1, Bénédicte Puissant-Lubrano, Pol-André Apoil
1Etablissement Français du sang Pyrénées-Méditerranée, Toulouse, France.
Journal of Clinical Immunology
|April 21, 2010
Summary
Fibroblasts, unlike bone marrow mesenchymal stem cells (BM-MSCs), lack multi-potency but share potent immunosuppressive capacities. These stromal cells suppress lymphocyte activation early in immune responses, offering therapeutic potential.
Area of Science:
- Immunology
- Stem Cell Biology
- Cell Biology
Background:
- Bone marrow mesenchymal stem cells (BM-MSCs) exhibit immunosuppressive properties.
- Adipose tissue-derived stem cells also possess immunosuppressive capacities.
- Stromal cells may generally possess immunosuppressive functions.
Purpose of the Study:
- To investigate the hypothesis that stromal cells, specifically fibroblasts, share immunosuppressive capacities with BM-MSCs.
- To compare the multi-potentiality, expandability, and immunomodulatory properties of human BM-MSCs and fibroblasts.
- To elucidate the mechanisms underlying fibroblast-mediated immunosuppression.
Main Methods:
- Comparative in vitro analysis of human BM-MSCs and fibroblasts.
- Assessment of multi-potentiality (differentiation into adipocytes and osteoblasts).
- Evaluation of immunomodulatory properties, including suppression of lymphocyte proliferation in mixed lymphocyte cultures and mitogen-induced assays.
- Analysis of specific molecular markers (CD106, CD10, CD26, collagen VII mRNA) and immunosuppressive factors (prostaglandin E2, IL-10, indoleamine 2,3-dioxygenase).
Main Results:
- Fibroblasts, unlike BM-MSCs, do not exhibit in vitro multi-potency for adipogenesis or osteogenesis.
- Fibroblasts express distinct membrane markers (CD106, CD10, CD26) and collagen VII mRNA compared to BM-MSCs.
- Fibroblasts strongly suppress lymphocyte proliferation, similar to BM-MSCs, independent of prostaglandin E2, IL-10, or indoleamine 2,3-dioxygenase.
- Fibroblast immunosuppression is effective even after depletion of CD8+ T lymphocytes, NK cells, and monocytes.
Conclusions:
- Fibroblasts, despite lacking multi-potency, possess significant immunosuppressive capabilities.
- Both fibroblasts and BM-MSCs exert immunosuppression by blocking early lymphocyte activation.
- This early immune suppression is evidenced by the down-regulation of granzyme B (GZMB) and IL2RA (CD25) expression.
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