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The proteome complement of Nicotiana tabacum Bright-Yellow-2 culture cells
Geoffrey Duby1, Hervé Degand, Anne-Marie Faber
1Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
Abstract:
The Nicotiana tabacum Bright-Yellow-2 (BY2) cell line is one of most commonly used plant suspension cell lines and offers interesting properties, such as fast growth, amenability to genetic transformation, and synchronization of cell division. To build a proteome reference map of BY2 cell proteins, we isolated the soluble proteins from N. tabacum BY2 cells at the end of the exponential growth phase and analyzed them by 2-DE and MALDI TOF-TOF. Of the 1422 spots isolated, 795 were identified with a significant score, corresponding to 532 distinct proteins.
