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A Fibrin-Enriched and tPA-Sensitive Photothrombotic Stroke Model
Published on: June 4, 2021
Genome response to tissue plasminogen activator in experimental ischemic stroke
Glen C Jickling1, Xinhua Zhan, Bradley P Ander
1Department of Neurology and M,I,N,D, Institute, University of California at Davis, Sacramento, California 95817, USA. gcjickling@ucdavis.edu
BMC Genomics
|April 22, 2010
Summary
Tissue plasminogen activator (tPA) influences blood gene expression, particularly immune cell modulation, in stroke models. Its effects depend on blood flow restoration, revealing non-fibrinolytic roles.
Area of Science:
- Biochemistry
- Immunology
- Neuroscience
Background:
- Tissue plasminogen activator (tPA) has known roles beyond fibrinolysis, including blood-brain barrier disruption.
- Investigating tPA's broader functions in the bloodstream is crucial for understanding stroke complications.
Purpose of the Study:
- To examine gene expression profiles induced by tPA in the blood of rats during ischemic stroke.
- To elucidate the non-fibrinolytic functions of tPA in the circulatory system.
Main Methods:
- Gene expression profiling was performed on blood samples from a rat model of ischemic stroke.
- Analysis focused on genes differentially expressed in response to tPA administration.
Main Results:
- tPA significantly altered the expression of 929 genes in rat blood (p
- Affected genes were primarily involved in immune cell modulation.
- tPA-induced gene expression varied with cerebral vasculature reperfusion status and differed from stroke-induced gene expression.
Conclusions:
- tPA modulates blood gene expression, impacting immune cells, in a manner contingent on vascular reperfusion.
- These findings highlight non-fibrinolytic tPA activities in the blood, aiding in understanding associated complications.
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