Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Urine Studies I: Urinalysis

Urine Studies I: Urinalysis

Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Precision Rehabilitation in Spinal Cord Injury: A Systematic Review of Omics Applications for Intervention Monitoring in Spinal Cord Injury.

Archives of rehabilitation research and clinical translation·2026

Same author

Higher disease reactivation risk in women after fingolimod withdrawal.

Acta neuropathologica communications·2026

Same author

Cardiac Biomarkers to Refine Pretest Probability for Coronary Obstruction and Predict Survival After Revascularization in Chronic Coronary Syndrome.

Journal of the American Heart Association·2026

Same author

<i>Trans</i>-eQTLs reveal the architecture of human gene regulatory networks.

medRxiv : the preprint server for health sciences·2026

Same author

Intraoperative text messaging to reduce parental stress during outpatient pediatric surgery: a randomized trial.

Pediatric research·2026

Same author

A genome-wide association meta-analysis of cholesterol synthesis intermediates identifies three associations for lanosterol.

EBioMedicine·2026

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Standardized preprocessing of urine for proteome analysis.

Georg Martin Fiedler¹, Uta Ceglarek, Alexander Leichtle

¹Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Medical Faculty, University Leipzig, Leipzig, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|April 22, 2010

Summary

This summary is machine-generated.

Standardizing urine pretreatment is crucial for reliable proteome and peptidome profiling. This study presents a validated protocol using magnetic bead separation and MALDI-TOF MS for reproducible biomarker discovery.

More Related Videos

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

Related Experiment Videos

Last Updated: Jun 13, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

Area of Science:

Biochemistry
Proteomics
Analytical Chemistry

Background:

Urine proteome/peptidome profiling offers potential for novel disease biomarker discovery.
Preanalytic variables significantly impact the reliability of proteome/peptidome analysis.
Standardization is essential for consistent and meaningful results in biomarker research.

Purpose of the Study:

To present a validated pretreatment protocol for human urine.
To standardize preanalytic modalities for reproducible peptidome profiling.
To facilitate consistent data generation for biomarker discovery and proteomic experiments.

Main Methods:

Development and validation of a pretreatment protocol for human urine samples.
Utilized magnetic bead (MB) separation technique.
Employed matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) for analysis.

Main Results:

The validated pretreatment protocol standardizes preanalytic variables.
Achieved reproducible peptidome profiling of human urine.
Enabled generation of consistent and reliable data for biomarker discovery.

Conclusions:

A validated pretreatment protocol is essential for reproducible urine peptidome profiling.
Magnetic bead separation combined with MALDI-TOF MS provides a reliable method.
This approach supports robust biomarker discovery and general proteomic studies.