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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Homogeneous analysis: label-free and substrate-free aptasensors
Bingling Li1, Shaojun Dong, Erkang Wang
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022 P. R. China.
Chemistry, an Asian Journal
|April 22, 2010
Summary
This review introduces label-free and substrate-free (LFSF) aptasensors for simplified homogeneous solution sensing. These advanced biosensors avoid common steps, streamlining operations for smart analysis.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Molecular Biology
Background:
- Biosensors traditionally require complex steps like labeling, separation, and immobilization.
- Aptamers offer high specificity and affinity as recognition elements in biosensing.
- Existing biosensing platforms often involve intricate procedures, limiting their practical application.
Purpose of the Study:
- To introduce and review label-free and substrate-free (LFSF) aptasensors.
- To highlight the advantages of LFSF aptasensors and aptamers in biosensing.
- To provide an overview of the most developed LFSF aptasensor systems.
Main Methods:
- Focus review of existing literature on LFSF aptasensors.
- Categorization of LFSF aptasensors into fluorescent, calorimetric, and hemin-aptamer-DNAzyme systems.
- Discussion of the homogeneous solution-based sensing mechanism.
Main Results:
- LFSF aptasensors enable sensing in homogeneous solutions, eliminating the need for labels and substrates.
- The review details three primary types of LFSF aptasensors: fluorescent, calorimetric, and hemin-aptamer-DNAzyme based.
- Sensing operations are significantly simplified by avoiding covalent labeling, separation, and immobilization steps.
Conclusions:
- Aptamers serve as ideal recognition elements for smart analysis in LFSF aptasensor designs.
- LFSF aptasensors represent a significant advancement in simplifying biosensing operations.
- The reviewed systems offer a promising direction for developing more efficient and user-friendly biosensors.
