Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

Creation of a functional heterogeneous vesicle array via DNA controlled surface sorting onto a spotted microarray.

Brigitte Städler¹, Marta Bally, Dorothee Grieshaber

¹Laboratory of Biosensors and Bioelectronics, Institute for Medical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, 8092 Zurich, Switzerland.

|April 23, 2010

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lipid packing contributes to the confinement of caveolae to the plasma membrane.

eLife·2026

Same author

METTL3 regulates exocytosis independently of m<sup>6</sup>A.

Science advances·2026

Same author

Salmonella lipopolysaccharide-containing supported lipid bilayers as platforms to study bacteriophage interactions.

FEBS letters·2026

Same author

Protocol for total internal reflection fluorescence microscopy image preprocessing and radial analysis of LFA-1 in T cell immune synapses.

STAR protocols·2026

Same author

A Lipid-Conjugation Strategy for Intracellular Reactive Oxygen Species Control in Hepatic Cells.

Angewandte Chemie (International ed. in English)·2026

Same author

Advancing physical virology through multiscale approaches-Insights from the 2025 FEBS|EMBO lecture course 'Physical Virology: across length scales'.

FEBS letters·2026

This study introduces a novel method for creating functional heterogeneous vesicle arrays using DNA-guided surface sorting, advancing drug discovery and screening technologies.

Area of Science:

Biotechnology
Surface Chemistry
Drug Discovery

Background:

Membrane protein microarrays are crucial for drug screening.
Existing methods face challenges in creating functional and selective arrays.

Purpose of the Study:

To develop a DNA-controlled surface sorting method for creating functional heterogeneous vesicle arrays.
To demonstrate the activity, selectivity, and low non-specific adsorption of these arrays.

Main Methods:

Utilizing DNA-tagged vesicles and streptavidin-biotinylated DNA complexes for surface sorting.
Employing a biomolecule- and cell-resistant surface coating.
Functionalizing vesicles with annexin A5 or encapsulating bovine serum albumin.

Main Results:

More Related Videos

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Related Experiment Videos

Last Updated: Jun 13, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

Successful creation of heterogeneous vesicle arrays at predefined spots.
Demonstration of sustained activity and selectivity of the functionalized vesicles.
Confirmation of resistance to non-specific adsorption of vesicles and proteins.

Conclusions:

The developed method enables the creation of functional heterogeneous vesicle arrays.
This approach holds significant potential for high-throughput drug screening and discovery.
The arrays exhibit excellent performance regarding activity, selectivity, and surface resistance.