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Updated: Jun 13, 2026

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
07:48

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging

Published on: June 7, 2014

Melting and interdigitation of microstructured solid supported membranes quantified by imaging ellipsometry.

Maja Gedig1, Simon Faiss, Andreas Janshoff

  • 1Institute of Physical Chemistry, University of Mainz, Welder Weg 11, 55128 Mainz, Germany.

Biointerphases
|April 23, 2010
PubMed
Summary

Researchers studied microstructured lipid bilayers, finding that ethanol can reversibly induce an interdigitated phase. Cholesterol was observed to abolish this interdigitation at higher concentrations.

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Area of Science:

  • Lipid bilayer membranes
  • Materials science
  • Physical chemistry

Background:

  • Microstructured lipid bilayers offer a platform for studying membrane properties.
  • Understanding phase transitions is crucial for membrane function and stability.

Purpose of the Study:

  • To investigate the phase transition behavior of microstructured lipid bilayers.
  • To determine the effects of temperature, ethanol, and cholesterol on membrane phase transitions.

Main Methods:

  • Noncontact imaging ellipsometry was used to analyze supported lipid bilayers.
  • Micromolding in capillaries created two-dimensional membrane compartments on silicon substrates.
  • Area expansion and bilayer thickness were measured as a function of various parameters.

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Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
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Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Published on: December 1, 2015

Related Experiment Videos

Last Updated: Jun 13, 2026

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
07:48

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging

Published on: June 7, 2014

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
09:38

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Published on: December 1, 2015

Main Results:

  • Ethanol was found to reversibly induce an interdigitated phase in dimyristoylphosphadiylcholine (DMPC) and dipentadecoylphosphatidylcholine (DiC(15)PC) membranes.
  • Cholesterol was observed to abolish interdigitation at higher concentrations.
  • The main phase transition temperature (T(M)) of DiC(15)PC showed biphasic behavior with varying ethanol concentration.

Conclusions:

  • Microstructured lipid bilayers can exhibit reversible phase transitions induced by ethanol.
  • Cholesterol content modulates the interdigitation of lipid bilayers.
  • The observed phase transition behavior is consistent with studies on lipid vesicles.