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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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Related Experiment Video

Updated: Jun 13, 2026

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

Published on: January 5, 2024

Immunoprecipitation to examine protein complexes.

Gregory J Pazour¹

¹Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.

Methods in Cell Biology

|April 23, 2010

Summary

Immunoprecipitation is a key cell biology technique for studying protein interactions in complex organelles like cilia. This guide covers essential methods and controls for successful immunoprecipitation experiments.

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Immunoprecipitation is a common method in cell biology.
It is particularly useful for studying protein interactions within complex cellular structures.
Eukaryotic cilia and flagella are examples of such complex organelles.

Purpose of the Study:

To provide a comprehensive overview of immunoprecipitation methods.
To detail the analysis of immunoprecipitation experiments.
To emphasize the importance of controls in immunoprecipitation.

Main Methods:

General immunoprecipitation techniques are discussed.
Methods for analyzing immunoprecipitation results are covered.
Essential control experiments are outlined.

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Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

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Last Updated: Jun 13, 2026

Immunoprecipitation with an Anti-Epitope Tag Affinity Gel to Study Protein-Protein Interactions

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Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

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Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Main Results:

The abstract does not contain specific results, but outlines the scope of the chapter.
It prepares the reader for understanding how to perform and validate immunoprecipitation assays.

Conclusions:

Immunoprecipitation is a powerful tool for dissecting protein complexes in organelles.
Proper methodology and controls are crucial for reliable results in immunoprecipitation studies.