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Related Experiment Video

Updated: Jun 13, 2026

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Published on: October 15, 2019

Analysis of redox-sensitive dynein components.

Ken-ichi Wakabayashi¹

¹Department of Biological Sciences, The University of Tokyo, Japan.

Methods in Cell Biology

|April 23, 2010

Summary

Cellular redox regulation by thioredoxins impacts flagellar beating. This study explores methods to assess and control axonemal protein redox states and identify redox-sensitive proteins.

Area of Science:

Cellular Biology
Biochemistry
Molecular Biology

Background:

Redox-based regulation is crucial for cellular functions.
Thioredoxins are key proteins in cellular redox regulation.
They are integral components of eukaryotic ciliary/flagellar axonemal dyneins.

Purpose of the Study:

To provide an overview of techniques for redox state assessment and modulation in axonemal proteins.
To describe methods for identifying redox-regulated proteins within the axoneme.
To highlight the role of thioredoxins in modulating flagellar motility.

Main Methods:

Review of established techniques for estimating and modulating the redox state of axonemal proteins.
Description of strategies for identifying redox-sensitive protein interactions within the axoneme.

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Last Updated: Jun 13, 2026

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Published on: October 15, 2019

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Published on: October 30, 2014

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy

Published on: February 14, 2016

Utilizing Chlamydomonas as a model organism to study dynein-associated thioredoxin function.

Main Results:

Dynein-associated thioredoxins interact with different proteins via disulfide bonds under varying redox conditions.
These interactions lead to observable changes in flagellar beating patterns.
Evidence suggests a direct link between redox state and flagellar motility regulation.

Conclusions:

Thioredoxin-mediated redox regulation is a significant factor in controlling flagellar function.
The presented techniques enable further investigation into redox-based mechanisms in cilia and flagella.
Understanding these mechanisms is vital for comprehending cellular motility and its regulation.