Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 13, 2026

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle
08:11

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Published on: March 31, 2013

Studying cilia in zebrafish.

Iain Drummond1

  • 1Department of Medicine, Harvard Medical School and Massachusetts General Hospital,Charlestown, Massachusetts 02129, USA.

Methods in Cell Biology
|April 23, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epithelial tubule interconnection driven by HGF-Met signaling in the kidney.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Epithelial tubule interconnection driven by HGF-Met signaling in the kidney.

bioRxiv : the preprint server for biology·2024
Same author

In Vivo Assessment of Laboratory-Grown Kidney Tissue Grafts.

Bioengineering (Basel, Switzerland)·2023
Same author

Twelve tips for using tactical decision games to teach non-technical skills.

Medical teacher·2021
Same author

Response to: Can gaming turn doctors into better tacticians?

Medical teacher·2016
Same author

From man to fish: What can Zebrafish tell us about ApoL1 nephropathy?

Clinical nephrology·2016
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Zebrafish embryos offer a powerful system for studying cilia development and function. Researchers can analyze gene roles in ciliogenesis using genetic tools and advanced imaging techniques in living or fixed embryos.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Cilia are crucial cellular structures involved in various biological processes.
  • Understanding the genetic basis of ciliogenesis and cilia function is essential for biomedical research.
  • Zebrafish (Danio rerio) present a unique model organism for studying these processes.

Purpose of the Study:

  • To highlight the utility of zebrafish embryos as a high-throughput system for analyzing genes involved in ciliogenesis and cilia function.
  • To describe methods for annotating the cilia proteome using zebrafish.

Main Methods:

  • Utilizing zebrafish mutants and gene knockdown via antisense morpholino oligos.
  • Employing high-quality in vivo imaging of cilia in living zebrafish embryos.

More Related Videos

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
09:33

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Published on: November 18, 2017

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
08:42

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Published on: February 19, 2016

Related Experiment Videos

Last Updated: Jun 13, 2026

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle
08:11

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Published on: March 31, 2013

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
09:33

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Published on: November 18, 2017

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
08:42

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Published on: February 19, 2016

  • Applying high-resolution wholemount imaging and electron microscopy for fixed cilia analysis.

    • Main Results:

    • Zebrafish embryos facilitate the identification and functional analysis of genes critical for cilia formation and function.
    • The system allows for efficient annotation of the cilia proteome through genetic and imaging approaches.
    • Established techniques enable detailed visualization and characterization of cilia.

    Conclusions:

    • Zebrafish embryos provide a versatile and efficient platform for genetic and functional studies of cilia.
    • The described methodologies support high-throughput analysis and detailed characterization of cilia biology.
    • This model system is invaluable for advancing our understanding of ciliopathies and related disorders.