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Lipid Absorption

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Related Experiment Video

Updated: Jun 13, 2026

Cholesterol Efflux Assay

Cholesterol Efflux Assay

Published on: March 6, 2012

Transintestinal cholesterol efflux.

Astrid E van der Velde¹, Gemma Brufau, Albert K Groen

¹Academic Medical Center, AMC Liver Center, Amsterdam, The Netherlands.

Current Opinion in Lipidology

|April 23, 2010

Summary

The intestine actively excretes cholesterol through transintestinal cholesterol efflux (TICE), a key process in maintaining cholesterol balance. This pathway in the small intestine is crucial for regulating body cholesterol levels and may offer therapeutic targets.

Area of Science:

Metabolic regulation
Cholesterol homeostasis
Gastrointestinal physiology

Background:

Cholesterol homeostasis involves complex metabolic pathways across multiple organs.
The liver has been the primary focus of research in cholesterol regulation.
The intestine's role in cholesterol transport is an emerging area of study.

Purpose of the Study:

To review recent advancements in understanding the intestine's role in cholesterol transport.
To highlight the significance of the intestine in cholesterol homeostasis.
To discuss emerging pathways of intestinal cholesterol excretion.

Main Methods:

Literature review of recent research on intestinal cholesterol transport.
Analysis of studies investigating cholesterol excretion mechanisms in the small intestine.

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High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

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The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

Published on: November 30, 2022

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Last Updated: Jun 13, 2026

Cholesterol Efflux Assay

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Published on: March 6, 2012

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

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Published on: January 7, 2019

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

Published on: November 30, 2022

Examination of evidence for active cholesterol secretion pathways.

Main Results:

Significant progress has been made in understanding intestinal cholesterol excretion systems.
The proximal small intestine actively secretes cholesterol via transintestinal cholesterol efflux (TICE).
In mice, TICE accounts for up to 70% of fecal neutral sterol excretion.

Conclusions:

The small intestine plays a critical role in regulating body cholesterol homeostasis.
Active absorption and excretion processes, including TICE, are key to sterol regulation in the intestine.
TICE presents a potential therapeutic target for reducing atherosclerosis.