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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

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Published on: November 16, 2019

Scattered light fluorescence microscopy: imaging through turbid layers.

Ivo M Vellekoop¹, Christof M Aegerter

¹Physik Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. ivo.vellekoop@physik.uzh.ch

|April 23, 2010

Summary

This study introduces a novel fluorescence microscope capable of imaging through thick, scattering tissues. It utilizes constructive interference of scattered light for subwavelength resolution imaging deep within turbid media.

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Area of Science:

Biomedical optics
Microscopy
Photonics

Background:

Microscopy penetration depth in turbid tissue is a significant challenge.
Imaging through scattering biological tissues remains difficult.

Purpose of the Study:

To demonstrate a novel fluorescence microscope for imaging through optically thick turbid layers.
To overcome the limitation of penetration depth in microscopic imaging.

Main Methods:

Utilizing scattered light, not straight-path propagation, for imaging.
Employing constructive interference to focus scattered laser light through turbid media.
Raster scanning the focus to image fluorescent structures.

Main Results:

Achieved subwavelength resolution imaging through optically thick turbid layers.
Successfully imaged microscopic fluorescent structures behind scattering layers.

Conclusions:

The developed fluorescence microscope offers a new approach for deep tissue imaging.
This technique enables high-resolution visualization of structures within scattering biological samples.