Effect of solvent polarity on the fluorescence quenching of biologically active 5BAMC by aniline in binary solvent mixtures
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Updated: Jun 13, 2026

Spatial Separation of Molecular Conformers and Clusters
Published on: January 9, 2014
Raveendra M Melavanki1, Raviraj A Kusanur, Jagadish S Kadadevaramath
1Department of Physics, M S Ramaiah Institute of Technology, Bangalore, 560054, Karnataka, India. melavanki74@rediffmail.com
Abstract:
The fluorescence quenching of 5, 6-benzo-4-azidomethyl coumarin (5BAMC) by aniline have been carried in different solvent mixtures of benzene (BN) and acetonitrile (AN) at room temperature by steady state fluorescence measurements. The quenching is found to be appreciable and a positive deviation from linearity was observed in the Stern-Volmer plot for the fluorophore in all the solvent mixtures. Various parameters for the quenching process have been determined using the extended S-V equation and have been found to be dependent on the solvent polarity. Further, with the use of finite sink approximation model, it is concluded that the bimolecular reactions quenching reactions are diffusion limited, and the distance parameter R' and mutual diffusion coefficient D are estimated independently.
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