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Updated: Jun 13, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Enhanced production of lipase by the thermophilic Geobacillus stearothermophilus strain-5 using statistical
Mohamed Sifour1, Taha I Zaghloul, Hesham M Saeed
1Department of Molecular and Cell Biology, Faculty of Science, University of Jijel, Ouled Aissa, Jijel, Algeria. sifourm@yahoo.fr
Abstract:
Statistically based experimental designs were applied to optimize the cultural conditions for the production of a glycerol-inducible lipase from the thermophilic Geobacillus stearothermophilus strain-5. The effect of nineteen culture conditions on enzyme production was evaluated using Plackett-Burman factorial design. Tween 80, K(2)HPO(4), glycerol and glucose were the most significant factors in improving enzyme production. The selected parameters were then further investigated using central composite design to define the optimal process conditions. Maximal enzyme activity (578 U/ml) was reached under the following conditions: glycerol, 2.24% (v/v); Tween 80, 0.76% (v/v); glucose, 0.76% (w/v) and K(2)HPO(4), 0.38% (w/v) which is about five folds the activity in basal medium. A verification experiment was carried out to examine model validation and revealed more than 98% validity.
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