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[Experimental study of combined staphylococcal, streptococcal and adenovirus infections in tissue culture]
Abstract:
Associated adenoviral, staphylococcus, and streptococcus infection was studied in the cultures of cells HEp-2 and PAO. Under conditions of monoinfection the cell culture largely inhibited the reproduction of staphylococci, and failed to influence the streptococci. In double and triple associated infections staphylococci overcame the inhibitory action of the cell culture. The pathogenic properties of cocci (plasma coagulation, hemotoxic properties) grown in the cell culture both under conditions of monoinfection, and in associations, failed to change. In double and triple associated infections adenoviruses did reproduce, but in lower titre than in monoinfection. Under conditions of mixed infection cocci penetrated and reproduced in the cell cytoplasm more intensively than in monoinfection. The cytopathic action was determined by viral associate, and was identical by its character to adenoviral monoinfection. A statistically significant increase in the activity of aldolase and transaminase enzymes was noted in mixed infection. The changes in the enzyme activity proved to depend on the character of the associations studied.
Insights
Mixed infections with adenovirus, Staphylococcus, and Streptococcus impact bacterial growth and viral replication in cell cultures. Mixed infections increased bacterial invasion and altered enzyme activity, demonstrating complex interactions.
Area of Science:
- Microbiology
- Cell Biology
- Virology
Context:
- Investigates the complex interactions between adenoviral, Staphylococcus, and Streptococcus infections within HEp-2 and PAO cell cultures.
- Examines the effects of monoinfection versus mixed infections on bacterial reproduction and viral replication.
Purpose:
- To elucidate the behavior of Staphylococcus and Streptococcus during monoinfection and mixed infections with adenovirus in cell cultures.
- To assess changes in bacterial pathogenicity and viral replication dynamics under associated infectious conditions.
- To analyze alterations in host cell enzyme activity (aldolase and transaminase) resulting from these infections.
Summary:
- Monoinfection with Staphylococcus was inhibited by cell cultures, while Streptococcus was unaffected. However, in mixed infections, Staphylococcus overcame this inhibition.
- Adenovirus replication was reduced in mixed infections compared to monoinfection.
- Cocci exhibited more intensive penetration and reproduction within the cell cytoplasm during mixed infections.
- Bacterial pathogenic properties remained unchanged, but cytopathic effects were primarily determined by the viral associate.
- Mixed infections led to a statistically significant increase in aldolase and transaminase enzyme activity, dependent on the specific microbial associations.
Impact:
- Provides insights into the complex interplay between viruses and bacteria within host cells.
- Highlights how mixed infections can alter microbial dynamics and host cell responses.
- Suggests potential implications for understanding disease pathogenesis and developing targeted therapeutic strategies.