Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcytosis of IgG01:15

Transcytosis of IgG

Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
Development of Immunocompetence01:22

Development of Immunocompetence

The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multi-glycomic tool for characterization of resistant starch.

Carbohydrate polymers·2026
Same author

LOESS-based normalization workflow for targeted HDL glycoproteomics in an Alzheimer's disease cohort.

RSC advances·2026
Same author

Selective Human-Milk-Inspired Antimicrobial Peptides for the Treatment of Bacterial Vaginosis.

Pharmaceutics·2026
Same author

Neonatal Enteric Infection Disrupts the Microbiota-Gut-Brain Axis Through Pattern Recognition Receptors and Altered Neuroimmune Signaling.

bioRxiv : the preprint server for biology·2026
Same author

Rapid design and production of Alpha-1 antitrypsin fusion proteins in a plant-based cell-free expression system.

Free radical biology & medicine·2025
Same author

Glycoside hydrolase-mediated glucomannan catabolism in <i>Segatella copri</i>, a target of microbiota-directed foods for malnourished children.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jun 13, 2026

Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets
08:12

Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets

Published on: September 6, 2019

Glycoprotein expression in human milk during lactation.

John W Froehlich1, Eric D Dodds, Mariana Barboza

  • 1Department of Chemistry, University of California, Davis, California 95616, USA.

Journal of Agricultural and Food Chemistry
|April 27, 2010
PubMed
Summary
This summary is machine-generated.

This study reveals new temporal changes in human milk protein glycosylation during early lactation. These findings highlight dynamic shifts in milk glycoproteome, impacting protein function and bioactive potential.

More Related Videos

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
10:17

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Published on: April 13, 2019

A Method for Targeted 16S Sequencing of Human Milk Samples
09:09

A Method for Targeted 16S Sequencing of Human Milk Samples

Published on: March 23, 2018

Related Experiment Videos

Last Updated: Jun 13, 2026

Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets
08:12

Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets

Published on: September 6, 2019

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
10:17

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Published on: April 13, 2019

A Method for Targeted 16S Sequencing of Human Milk Samples
09:09

A Method for Targeted 16S Sequencing of Human Milk Samples

Published on: March 23, 2018

Area of Science:

  • Biochemistry
  • Proteomics
  • Human Milk Studies

Background:

  • Milk proteins are extensively studied, but their post-translational modifications (PTMs) during lactation remain under-explored.
  • PTMs, especially glycosylation, significantly affect protein properties and the bioactivity of their degradation products in the infant gut.

Purpose of the Study:

  • To investigate temporal variations in the expression and glycosylation of human milk proteins throughout lactation.
  • To identify changes in the human milk glycoproteome during the early stages of lactation.

Main Methods:

  • Analysis of human milk samples collected during the first 10 days of lactation.
  • Proteomic techniques to assess protein expression levels.
  • Glycosylation analysis to determine modifications on milk glycoproteins.

Main Results:

  • Discovery of previously unknown temporal variations in both expression and glycosylation of the human milk glycoproteome.
  • Demonstration of dynamic glycosylation changes in lactoferrin within the first 10 days of lactation.
  • Observed variations in expression or glycosylation for other key whey proteins like tenascin and bile salt-stimulated lipase.

Conclusions:

  • Human milk protein expression and glycosylation exhibit significant temporal dynamics during early lactation.
  • These dynamic changes in the glycoproteome may influence the functional properties and bioactivity of milk proteins.
  • Further research into lactation-induced PTM variations is warranted to understand their full impact.