Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Induction of Cerebral Arterial Gas Embolism in Rat

Induction of Cerebral Arterial Gas Embolism in Rat

Published on: October 18, 2024

A rat model for cerebral air microembolisation.

Tibo Gerriets¹, Maureen Walberer, Max Nedelmann

¹Department of Neurology, Experimental Neurology Research Group, Justus-Liebig-University Giessen, Germany.

Journal of Neuroscience Methods

|April 27, 2010

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SGLT2 inhibitors in metabolic dysfunction-associated steatotic liver disease (MASLD/NAFLD): Mechanisms, clinical benefits, and outcomes.

Advances in clinical and experimental medicine : official organ Wroclaw Medical University·2026

Same author

Preoperative stress markers as predictors of postoperative neuropsychological disorders in cardiac surgery patients: protocol for a single-centre prospective observational study (CAVALIR).

BMJ open·2026

Same author

The effect of frailty on hospitalisation trajectories in adults aged 65 and older across 10 European countries: a 14-year longitudinal analysis from the Survey of Health, Ageing, and Retirement in Europe (SHARE).

European journal of ageing·2026

Same author

Ultrasound measurement of optic nerve sheath diameter pre and post lumbar puncture for prediction of postdural puncture headache.

Scientific reports·2026

Same author

Neurocognition and health-related quality of life in patients randomized to surgical or transcatheter aortic-valve replacement.

Clinical research in cardiology : official journal of the German Cardiac Society·2026

Same author

Endoscopic Management of Acute Gastrointestinal Bleeding From a Gastric Lipoma: A Case Report, Literature Review, and Treatment Recommendation With Reference to ESGE Guidelines.

Case reports in gastrointestinal medicine·2025

A new method allows precise injection of controlled air bubbles into rat carotid arteries, aiding research into subtle cerebral air microembolisation (CAM) and its neurological effects.

Area of Science:

Biomedical Engineering
Neurology
Experimental Medicine

Background:

Subtle cerebral air microembolisation (CAM) is a common complication of medical procedures like open heart surgery and angiography.
CAM can lead to neurological and neuropsychological deficits, necessitating reliable animal models for study.
Current models lack the ability to control the size and number of air bubbles for precise pathophysiology evaluation.

Purpose of the Study:

To develop and validate a method for producing and injecting gas bubbles of defined diameter and number into the carotid artery of rats.
To quantify the number of injected gas microemboli using advanced imaging and software.
To establish a reproducible animal model for investigating the effects of controlled air embolism.

Main Methods:

More Related Videos

Establishment of a Minimally Invasive Rat Model of Pulmonary Embolism Using Autologous Blood Clots

Establishment of a Minimally Invasive Rat Model of Pulmonary Embolism Using Autologous Blood Clots

Published on: October 25, 2024

Related Experiment Videos

Last Updated: Jun 13, 2026

Induction of Cerebral Arterial Gas Embolism in Rat

Induction of Cerebral Arterial Gas Embolism in Rat

Published on: October 18, 2024

Establishment of a Minimally Invasive Rat Model of Pulmonary Embolism Using Autologous Blood Clots

Establishment of a Minimally Invasive Rat Model of Pulmonary Embolism Using Autologous Blood Clots

Published on: October 25, 2024

Development of a technique for generating gas bubbles with a consistent diameter (160 microm).
Injection of varying numbers of these bubbles (0, 50, 100, 400, 800) into the carotid artery of rats.
Digital quantification of injected microemboli using high-speed optical imaging and custom software.
Assessment of neurological deficits and brain infarctions (TTC staining) in a pilot study.

Main Results:

The method consistently produced bubbles with a mean diameter of 159.95 ± 9.25 micrometers.
The number of injected bubbles was accurately quantified.
Preliminary data showed a dose-dependent relationship between the number of injected bubbles and the incidence of neurological deficits and brain infarctions.
Neurological deficits were observed even in animals without overt infarcts, suggesting subtle brain damage.

Conclusions:

The presented method enables the controlled injection of air bubbles with defined number and diameter into the cerebral arteries of rats.
This technique provides a valuable tool for advancing research into the pathophysiology of air embolism.
The model facilitates a better understanding of subtle brain damage caused by air microemboli in medical interventions.