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Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Putative stem cells in human dental pulp with irreversible pulpitis: an exploratory study.

Zhengyan Wang¹, Jian Pan, John T Wright

¹Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA. zhengyan@umich.edu

Journal of Endodontics

|April 27, 2010

Summary

Clinically compromised dental pulp may contain stem cells with regenerative potential. These cells show some stem cell properties, suggesting a possible new source for dental pulp therapy.

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Area of Science:

Dental Pulp Biology
Stem Cell Research
Regenerative Dentistry

Background:

Human dental pulp stem cells (DPSCs) from healthy teeth are well-characterized.
The presence and properties of stem cells in teeth with irreversible pulpitis are largely unknown.

Purpose of the Study:

To investigate stem cell-like properties of cells from clinically compromised dental pulp.
To compare these cells with those from healthy dental pulp.

Main Methods:

Isolation of pulp cells from healthy and irreversibly inflamed teeth.
Assessment of cell proliferation, STRO-1 marker expression, and odonto-osteogenic differentiation potential.

Main Results:

Cells from diseased pulp showed reduced colony formation and proliferation.
STRO-1 expression and osteogenic/dentin sialophosphoprotein differentiation were similar between groups.
Enriched STRO-1 positive cells from diseased pulp maintained differentiation capacity.

Conclusions:

Clinically compromised dental pulp harbors cells with certain stem cell properties.
These findings suggest potential endogenous multipotent cells for pulp regeneration.
Further research is needed to fully characterize these cells for therapeutic applications.