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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
Role of proline and hydroxyproline in N-nitrosamine formation during heating in cured meat
G Drabik-Markiewicz1, K Van den Maagdenberg2, E De Mey2
1Laboratory for Food Chemistry and Meat Technology, KaHo Sint-Lieven, Technology Campus, Gebroeders Desmetstraat 1, 9000 Gent, Belgium; The University of Silesia, Institute of Chemistry, 9 Szkolna Street, 40-006 Katowice, Poland.
Abstract:
N-Nitrosamines are formed in a multi-step reaction of nitrite with free amino acids and amines in the meat products. The aim of this study was to determine the role of proline and hydroxyproline in N-nitrosamines formation during heating of cured meat. A lean meat model was used with different nitrite concentrations (0, 120, and 480mg/kg), and addition of proline and hydroxyproline (1000mg/kg), followed by heating at different temperatures. Volatile nitrosamines were analyzed with GC-TEA. The nitrosamine content never exceeded 10μg/kg and stayed
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