Related Experiment Video
Updated: Jun 13, 2026

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
Published on: February 27, 2014
Effect of cooking on protein oxidation in n-3 polyunsaturated fatty acids enriched beef. Implication on nutritional
Ph Gatellier1, A Kondjoyan, S Portanguen
1INRA, UR370, QuaPA, F-63122 St Genès Champanelle, France. pgatel@clermont.inra.fr
Abstract:
The effect of cooking on protein oxidation was investigated in M. Longissimus thoracis of eight Normand cows fed during a 100 days finishing period with two different diets: a conventional diet (concentrate/straw based diet) and a diet rich in n-3 polyunsaturated fatty acids (PUFAs), obtained by addition to the conventional diet of a mixture of extruded linseed and extruded rapeseed. After 11 days storage, at 4 degrees C under vacuum, meat was cooked by applying jets of steam. Three experimental heating treatments were tested: two with constant surface temperatures of 65 and 96 degrees C during 300s, and one with a continuous increasing surface temperature up to 207 degrees C. Protein oxidation was evaluated by the measurement of carbonyls, aromatic amino acids, and free thiols content. The formation of Schiff bases due to the reaction of proteins with aldehydic products of the lipid oxidation was also evaluated. Cooking resulted in a significant increase of carbonyl groups and Schiff bases as well as a significant degradation of tyrosine and tryptophan. Nevertheless, enrichment of the animal diet in n-3 PUFAs had minor effects on protein oxidation induced by cooking which are unlikely to be of nutritional significance.
More Related Videos
06:00Optimization of the Epimedii Folium Mutton-Oil Processing Technology and Testing Its Effect on Zebrafish Embryonic Development
Published on: March 17, 2023
07:46Exploring the Longissimus Muscle: Unraveling its Correlation with Meat Quality in Bos indicus and Crossbred Bulls
Published on: July 12, 2024
Related Concept Videos
Radical Autoxidation
Protein Denaturation
Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids
2° Amines to N-Nitrosamines: Reaction with NaNO2
Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide
Protein Modifications in the RER
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.